Figure 7.

Western blot analysis of Brn-3a(l) expression in (a) ovarian cancer cell lines and (b) tumor cells from ascites of cancer patients. Cell lysates (25 μg) of (a) ovarian cancer cell lines (OVCA433, OVCA429, SKOV3 and 2008) and (b) tumor cells isolated from the ascites of patients with advanced-stage ovarian cancer (As2, As4, As9, As11) were prepared and loaded on 10% SDS-PAGE gels as described in the Methods and Material. Blots were probed with mouse monoclonal Brn-3a(l) antibody, stripped and re-probed with mouse monoclonal β-actin antibody as an internal protein loaded.