Figure 5.

Protein expression of AKT and phopsho-AKT (pAKT) in xenograft tumors. (A) After SKOV-3 cells were treated with antibodies for 12 h, the cell lysates were analyzed. pAKT (Ser⁴⁷³) expression educed upon treament withchA21 or trastuzumab treatment and more dramatic reduction was observed with chA21 plus trastuzumab treatment. The levels of total AKT protein were not altered significantly upon various interventions. (B) The ratios of p-AKT/AKT were calculated and the values were shown as ratios compared with control, which was arbitrarily taken as 1.0. *, P < 0.01 compared with control. **, P < 0.01 compared with chA21 or trastuzumab alone.