Research
Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter
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* Corresponding author: Kenneth W Escudero kfkwe00@tamuk.edu
- † Equal contributors
1 Department of Biological and Health Sciences, Texas A&M University- Kingsville, Kingsville, TX, USA
2 Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA
3 Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA
Journal of Ovarian Research 2010, 3:4 doi:10.1186/1757-2215-3-4
Published: 18 February 2010Additional files
Additional file 1:
Pairwise analysis of differentially expressed genes in KK1 cells. Group NA: mock vs. VP16.
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Additional file 2:
Pairwise analysis of differentially expressed genes in KK1 cells. Group NR: mock vs. Mad.
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Additional file 3:
Pairwise analysis of differentially expressed genes in KK1 cells. Group RA: Mad vs. VP16.
Format: XLS Size: 3.3MB Download file
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Additional file 4:
Comprehensive listing of potential Foxl2 target genes of known function generated by microarray analysis. Group designations: (-) decreased fold expression; (+) increased fold expression. NA = Normal: Activated (mock transfected compared to Foxl2-VP16 transfected). NR = Normal: Repressed (mock transfected compared Foxl2-Mad transfected). RA = Repressed: Activated (Foxl2-Mad compared to Foxl2-VP16). E16 = mouse embryonic stage day 16. E13 = mouse embryonic stage day 13. P0 = mouse birth stage.
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Additional file 5:
Combined analysis of differentially expressed genes in KK1 cells. Normal: mock transfected. Activated: Foxl2-VP16 transfected. Repressed: Fodl2-Mad transfected.
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Additional file 6:
Analysis of differentially expressed genes in E13. Wild type vs. Foxl2 knockout.
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Additional file 7:
Analysis of differentially expressed genes in E16. Wild type vs. Foxl2 knockout.
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Additional file 8:
Analysis of differentially expressed genes in P0. Wild type vs. Foxl2 knockout.
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Additional file 9:
Comparison of differentially expressed genes in KK1 cells to E13, E16, and P0. Sheet 1 lists 225 genes common to KK1 and E13. Sheet 2 lists 211 genes common to KK1 and E16. Sheet 3 lists 325 genes common to KK1 and P0. Sheet 4 lists 5 genes common to all 3 groups. Sheet 5 lists 22 common genes. Sheet 6 lists 29 common genes. Sheet 7 lists 21 common genes. Sheet 8 lists 155 genes unique to KK1 & E16. Sheet 9 lists 177 genes unique to KK1 & E13. Sheet 10 lists 270 genes unique to KK1 & P0.
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Additional file 10:
Potential Foxl2 target genes validated by comparison to data from other microarray studies. Gene Functions were obtained from the Ovarian kaleidoscope data base1, NCBI RefSeq2, and UniProtKB/Swiss-Prot3. All genes listed that do not have an asterisk after the gene symbol are common to our study and the in vivo in mouse ovary study only [7]. Genes denoted by a single asterisk* are common to our study and the human KGN cell line only [6]. Those indicated by ** are common to all three studies. The StAR*** and GnRHR*** genes were found to be Foxl2 regulated in our study and do not appear in the KGN [6] or in vivo [7] studies. Fold change numbers indicate relative gene expression levels. Group designations: (-) decreased fold expression; (+) increased fold expression. NA = Normal: Activated (mock transfected compared to Foxl2-VP16 transfected). NR = Normal: Repressed (mock transfected compared Foxl2-Mad transfected). RA = Repressed: Activated (Foxl2-Mad compared to Foxl2-VP16). E16 = mouse embryonic stage day 16. E13 = mouse embryonic stage day 13. P0 = mouse birth stage.
Format: XLS Size: 239KB Download file
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