Figure 3.

HER expression reprogramming in ovarian carcinoma-derived cell lines following long-term trastuzumab treatment. Immunoblot of epidermal growth factor receptor (EGFR), HER2, HER3, and HER4 expression in parental vs. T100 ovarian carcinoma cell lines A1847, IGROV-1, OVCAR-7, and SKOV-3. Lysates of parental and T100 subclones, normalized for protein content, were probed following SDS-PAGE with α-EGFR, -HER2 -HER3, -HER4, and -tubulin. Note, enhanced chemiluminescent exposures were calibrated for each cell line to allow for accurate assessment of individual HER receptors; the HER expression patterns presented here are therefore not comparable between lanes.