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Open Access Research

Establishment of a new representative model of human ovarian cancer in mice

Jianjun Zhang135, Xinlian Chen145, Gang Shi3, Xiaoyan Xie145, Hongqian Liu145, Xuemei Zhang145, Yi Lai4, Yan Zuo3, Zhong Chen6*, Shanling Liu2345* and He Wang1345*

Author Affiliations

1 Laboratory of Genetics, West China Institute of Maternal and Child Health, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China

2 Laboratory of Cell and Gene Therapy, West China Institute of Maternal and Child Health, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China

3 Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, 610041, P. R. China

4 Prenatal Diagnosis Center of Sichuan Province, West China Second University Hospital, Sichuan University, Chengdu, 610041, P. R. China

5 Key Laboratory of Obstetrics, Gynecology, Pediatric Diseases and Birth Defects of Ministry of Education, Chengdu, 610041, P. R. China

6 Division of Medical Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA

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Journal of Ovarian Research 2013, 6:9  doi:10.1186/1757-2215-6-9

Published: 6 February 2013

Abstract

Background

Intraperitoneal (i.p.) models that accurately mimic the feature behavior of human ovarian cancer are required to investigate the pathology and therapeutics of the disease. However, established i.p. models which are well-characterized and reliable are few. The purposes of this study are to establish a representative mice i.p. model of the disease and to analyze the consequent pathology.

Methods

Fresh tumor cells fiom the ascites of patient were injected into female NOD/SCID mice intraperitoneally. Histology, Cytogenetic, immunohistochemistry,tumor markers of CA125,AFP, CA-199 and CEA were used to analyze the model.

Results

The mice developed marked abdominal distention within 6 months after inoculated with tumor cells from a patient with epithelial ovarian carcinoma. The mice developed clinically evident intraperitoneal tumors and massive ascites containing numerous tumor cells in clumps. CA125 level in our model was high in both serum and ascites supernatants, while levels of other tumor markers, such as AFP, CA-199 and CEA, were normal. Cytogenetic analysis and immunohistochemical staining confirmed its characteristics resembling human epithelial ovarian tumor.

Conclusions

The model described in this paper accurately mimics the features of ovarian tumor, which may be useful for evaluation of new therapeutics.

Keywords:
Human; Ovary carcinoma; Animal model; CA125; Intraperitoneal