As early as 1969, ovarian tumors were induced by direct application of chemical carcinogens 4. While 7,12-Dimethylbenz(a)anthracene (DMBA) had been used in 1970 to induce tumorigenesis in guinea pigs 5, a DMBA-coated suture was used in 1984 to induce ovarian tumorigenesis with only one of thirty-five mice developing an epithelial carcinoma 6. However, despite these discouraging results, Nishita et al. 7 replicated this experiment by directly applying DMBA to the rat ovary using a coated suture. Nearly fifty percent of the rats developed ovarian tumors in 36 weeks, most of which were carcinomas. Unfortunately, DMBA also stimulated the epithelial surface of the fallopian tube, endometrium, and cervix to induce neoplastic transformation.

Other chemical carcinogens used to induce ovarian tumorigenesis include 20-methylcholanthrene, 1,3-butadiene, formic acid 2- [4-(5-nitro-2-furyl)-2-thiazolyl]hydrazide, a nitrofuran antibiotic, and N-methyl-N'-nitrosourea, a direct-acting alkylating agent 8910. To date, chemical carcinogens have not been associated with OC etiology 11.

Syngeneic models combine in vitro and in vivo methods to generate a tumor model. Briefly, mouse ovarian surface epithelial (MOSE) cells are isolated from the ovaries of virgin wildtype mice and cultured in vitro before transplantation into recipient mice 12. Development of the mouse model was predicated on the work by Godwin et al. 13 and Testa et al. 14 on the spontaneous transformation of surface epithelial cells isolated from rats.

Roby et al. 12 established the technique of isolating and culturing MOSE cells, showing that MOSE cells can spontaneously transform in vitro with repeated passages and have tumorigenic capacity as they formed tumors and hemorrhagic ascitic fluid upon injection into athymic and C57Bl/6 receipt mice. This technique has been used by numerous investigators for subsequent studies 151617181920.

Perhaps one of the most revealing MOSE studies was conducted by Roberts et al. 15, who compared the alterations of the actin cytoskeleton as well as expression of cellular adhesion proteins versus the number of passages to study the progression of ovarian carcinogenesis, showing that MOSE cells spontaneously transform with repeated passages. Late passage cells injected intraperitoneally into immunocompetent C57BL6 mice formed tumors in numerous organs, showing the transformation from a premalignant to a highly malignant phenotype with downregulation of E-cadherin and connexin-43.

Greenaway et al. 16 injected a spontaneously tumorigenic MOSE cell line, ID8, into the ovarian bursal cavity of C57Bl6 mice. The ID8 cells formed direct contact with the ovarian stroma, resulting in primary tumor formation, secondary peritoneal carcinomatosis, and extensive ascites fluid production between 80 to 90 days post-exposure. The cytological and architectural features resembled serous carcinoma. Interaction between ID8 cells and the ovarian stroma resulted in increased expression of proliferative and survival markers, including phosphorylated Akt, proliferating cell nuclear antigen (PCNA), and Bcl-2. Vascular endothelial growth factor (VEGF) levels were also increased in the serum and ascitic fluid. In conjunction, the pro-apoptotic factor Bax was decreased. The study supports the theory that the ovarian surface epithelium (OSE) can undergo invaginations and form inclusion cysts capable of undergoing neoplastic transformation 21.

One of the first reports to test genetic changes was made by Orsulic et al. 22, who used an avian retroviral delivery system. Transgenic mice were established to express the TVA virus receptor making them susceptible to infection to a subgroup of replication-competent avian leukosis viral-derived vectors (RCAS), thus allowing for the introduction of oncogenes that would integrate newly reverse-transcribed DNA into the host genome and allow long-term expression. The TVA receptor was placed under control of the keratin 5 promoter to direct expression to the ovarian epithelium or under control of the β-actin promoter to direct expression to all cells of the ovary. TVA-transgenic mice were crossbred with p53^-/-mice to generate TVA/p53^-/-, which were used to study the oncogenes c-myc, K-ras, and Akt individually and in combination. However, the keratin 5 promoter is constitutively active in the basal layer of stratified and simple epithelia in several organs 23; therefore, it was necessary to isolate the expression of the virally delivered oncogenes. The ovaries were removed from the TVA/p53^-/- mice, cultured, and infected in vitro before introduction into recipient mice either by subcutaneous or intraperitoneal injection or by transplantation under the ovarian bursa. Once infected, the mammalian cells would not produce detectable levels of infectious viral particles, which limited spreading to the surrounding tissue. Introduction of any two oncogenes in keratin 5-TVA/p53^-/- ovarian cells was sufficient to drive tumorigenesis. While providing valuable insight into the genetics of tumorigenesis, this methodology is cumbersome at best. Because transplantation and in vitro manipulation are required, it is not possible to generate a stable transgenic line with an inheritable form of EOC.

Connolly et al. 24 used a novel approach to target simian virus 40 T antigen (SV40 TAg) to the epithelial ovarian surface by using the Mullerian Inhibitory Substance Type II Receptor (MISIIR) promoter. The Mullerian duct in the 8-week old embryo gives rise to female reproductive organs, including the fallopian tubes, uterus, and upper vagina. By linking the MISIIR promoter to the SV40 TAg, they were able to target expression of SV40 TAg to the epithelium of the female reproductive tract by microinjection of this construct into the male pronucleus of 0.5-day old embryos to generate transgenic animals. While 18 of 36 (50%) transgenic mice developed bilateral ovarian tumors resembling serous carcinomas by 6 to 13 weeks of age, the aggressiveness of this formation inhibited reproduction, making it extremely difficult to establish a transgenic line via the female founders. Two individual transgenic mice also developed a uterine mass and enlarged polycystic kidneys, respectively, possibly due to recombination events during transgenic mouse production. Not unexpectedly, 7 of 25 (28%) transgenic animals developed testicular cancer. Intrapleural invasion of tumors into the omentum, the mesentery, and visceral and parietal pleura was also observed, possibly due to the invasiveness of the ovarian tumors. However, SV40 TAg is not known to be a genetic contributor to ovarian carcinogenesis 32526. Yet despite these limitations, this model has been used for further experiments by establishing a transgenic line through the male founder 2728.

Models that require either ex vivo manipulation or expression of a transgene during embryonic development do not accurately represent human EOC, which tends to be spontaneous in post-menopausal women. In an effort to mimic spontaneous EOC development, Flesken-Nikitin et al. 29 obtained mice from Anton Berns 3031 with LoxP sites containing p53 and Rb alleles to assess gene inactivation in the initiation of EOC. Mice were homozygous for the mutation and crossbred to generate p53^floxP/floxPRb1^floxP/floxP. To assess the efficiency of Cre recombinase (Cre) expression derived by the cytomegalovirus (CMV) promoter, the ovaries were removed and cultured prior to exposure to adenovirus infection. Adenoviruses carrying CMV-enhanced fluorescent green protein (AdCMVEGFP) were used as a control against adenoviruses carrying CMV-Cre (AdCMVCre). Administration of AdCMVCre resulted in increased cell proliferation assessed by BrdU incorporation. To detect the feasibility in targeting the ovarian bursal cavity in the mouse, AdCMVEGFP was administered. It was detected only in the OSE for 21 days, as expected with a transient adenovirus infection. As a result of both p53 and Rb inactivation, 33 of 34 mice succumbed to ovarian tumors at a median of 227 days. However, administration of an adenovirus to achieve the desired results is cumbersome without generating a reproductive line that would spontaneously form tumors. Targeting the ovarian bursal cavity is difficult at best, making this model not a feasible choice for large-scale applications.

While the previous models developed tumors resembling human serous adenomas, Dinulescu et al. 32 generated mice that have a transcriptionally silent oncogenic allele of K-ras (LSL-K-ras^G12D/+) as first developed by Tyler Jacks 333435, which can be conditionally expressed through administration of an adenovirus containing Cre. While the LSL-K-ras^G12D/+ mice formed benign endometrosis-like lesions and benign lesion within the OSE upon K-ras activation, the mice did not form ovarian carcinomas. However, when the LSL-K-ras^G12D/+ mice were crossed with PTEN^loxP/loxP mice, they developed invasive primary ovarian endometrioid adenocarcinomas (OEA), a subtype of EOC, suggesting that phosphate and tensin homologue deletion on chromosome 10 (PTEN) plays a role in tumorigenesis when combined with other oncogenes. This finding is consistent with PTEN deletion or mutation in other cancer types including endometrium, breast, thyroid, intestines, prostate, lung, liver, and T-cell lymphomas 3637383940. Concurrent K-ras and PTEN mutations have also been found in complex endometrial hyperplasias, the precursor type of uterine endometrioid adenocarcinomas 41.

Wu et al. 42 used similar methods to conditionally delete PTEN and adenomatous polyposis coli (APC) tumor suppressor gene upon administration of an adenovirus carrying Cre. APC has been shown to regulate Wnt/β-catenin signaling 43. Wu et al. cross-bred PTEN^loxP/loxP with APC^loxP/loxP transgenic mice to determine if there was an interaction between the two pathways. The PTEN^-/-APC^-/- animals developed tumors within 6 weeks upon inactivation, with death occurring at 19 weeks. These tumors resembled human OEA, with increased signaling through Akt. Loss of E-cadherin and cytokeratins indicated that these tumors were undergoing epithelial-mesenchymal transition (EMT), which is consistent with Wnt/β-catenin and PI3K/Akt activation 4445. Both the studies by Dinulescu et al. 32 and Wu et al. 42 rely on adenovirus administration and are therefore subject to the same limitations.

Chodanker et al. 46 crossbred mice with follicle stimulating hormone (FSH) receptor promoter fused to Cre recombinase (FSHR-Cre) to mice carrying Brca1^loxP/loxP to conditionally knockout Brca1 in the granulosa cells. Loss of Brca1 resulted in multiple cyst formation in 40 of 59 animals (58%) attached to the ovary wall and interior or exterior surface of the uterine horns, which resembled human serous cystademonas, the benign form of ovarian serous carcinomas. One animal formed a solid tumor. Although the FSHR promoter targeted the granulosa cells, the cysts resembled an epithelial morphology as they expressed keratins.

Clark-Knowles et al. 47 used Brca1^loxP/loxP mice, which upon administration of AdCre would remove introns 5 through 13 (Brca1^Δ55-13). Conditional deletion of Brca1 resulted in morphological changes, such as surface epithelium hyperplasia and formation of inclusion cysts, which was not due to increased proliferation. The incidence of these changes increased over time as observed from 60 days post-infection to 240 days. Interestingly, the genes involved in cancer initiation and progression p53 48, E-cadherin 49, and Collagen IV 50 were altered in Brca1^Δ55-13 ovaries compared to other tumor models. In Brca1^Δ55-13 ovaries, p53 was absent compared to SV40-induced tumors. E-cadherin was also downregulated, consistent with preneoplastic transformation. Collagen IV expression was found in the basement membrane, regardless of morphological changes of the OSE.

Building on the report by Connolly et al. 24, El-Naggar et al. 51 used the MISIIR promoter linked to the pituitary tumor-transforming gene (PTTG) to target expression to the OSE. This construct was microinjected into the male pronucleus of CD2F1 embryos to produce transgenic founders. The founders were crossbred with wildtype animals to produce the F1 generation. Positive male and female F1's from the same line were crossbred to produce the F2 generation. While the transgenic females failed to generate any visible tumors, there was an increase in the corpus luteum mass in the transgenic ovaries, which was accompanied by the increase in serum luteinizing hormone (LH) and testosterone levels. The transgenic females also displayed a generalized hypertrophy of the endometrium. This study showed that by using the MISIIR promoter, 3 different tissues could be targeted: OSE, granulosa cells, and pituitary.

More recently, Liang et al. 52 used the MISIIR promoter to drive expression of murine phosphatidylinositol 3-kinase catalytic subunit p110-alpha (PIK3CA) in transgenic mice. Although over-expression of PIK3CA resulted in increased phosphorylated Akt as its downstream target and in OSE hyperplasia, after 18 months post-birth of the F1 generation, tumorigenesis did not occur. Interestingly, the authors cultured isolated ovaries from non-transgenic mice and co-transfected them with both PIK3CA and mutant K-ras or c-myc to assess OSE transformation in vitro. Concurrent over-expression of PIK3CA and mutant K-ras led to increased anchorage-independent growth of cultured OSE cells. Liang et al. 52 acknowledged that producing a "bigenic" animal by crossbreeding the transgenic PIK3CA mouse with a transgenic mutant K-ras remains a technical challenge because mutant K-ras animals develop tumors that inhibit reproduction. However, they suggested that a Cre-lox system of K-ras expression may provide an alternative method of generation.

Granulosa cell tumors (GCT) represent 2-5% of all OCs 53 arising from the granulosa cells of the ovary, which are responsible for estradiol production. Therefore, GCT are also called sex cord-stromal tumors. One of the first GCT models was produced by Kananen et al. 54, who fused the inhibin α-subunit promoter to SV40 TAg to generate transgenic founders. Three lines were established from these founders with all transgenic offspring developing GCT in two of the lines: 14/14 animals in one line and 22/22 animals in another. The granulosa cells still maintained their receptors, making them responsive to gonadotrope stimulation. SV40 TAg mRNA expression was found in the gonads, adrenal glands, pituitary, and brain indicating leakiness of the inhibin α-subunit promoter.

Nilson et al. 55 generated a GCT tumor model through chronic hyperstimulation of LH by fusing the β-subunit of LH containing a carboxy-terminal peptide of human chorionic gonadotropin β subunit to a bovine inhibin α-subunit promoter (α-LHβCTP) to extend its half-life and target gonadotrope cells. As a result of the constant LH stimulation, the ovary became anovulatory from its inability to respond to the necessary LH surge. While the animals could be super ovulated, the pregnancy failed at mid-gestation. Females also displayed a reduction in the amount of primordial follicles with an increase in large hemorrhagic follicles. By 5 months of age, the females developed GCT and pituitary hyperplasia, dying shortly thereafter due to bladder atony and kidney failure.

Selvakumaran et al. 56 isolated a new promoter to determine specificity to the ovary by using repetitive retrovirus-like elements in the rat genome, termed ovarian-specific transcription units (OSTUs). The U3 region of the OSTUs was cloned and renamed ovarian-specific promoter-1 (OSP-1). OSP-1 was then used by Garson et al. 43 to drive expression of SV40 TAg (OSP-TAg). While successfully producing both male and female founders, many females either failed to reproduce or the offspring failed to develop tumors despite high levels of expression of TAg. Two of the three female founders developed GCT, but expression was not restricted to the ovary as osteosarcomas formed in the liver and lung. The thymus also showed enlargement demonstrating that OSP-1 was sufficiently leaky. Male founders also expressed TAg in a variety of tissues including testes, liver, and lung, but failed to produce any tumors.

Boerboom et al. 57 showed that constitutive activation of β-catenin in granulosa cells of transgenic mice (Catnb^flox(ex3)Amhr2^cre/+) produced GCT. Cre knocked into the anti-Mullerian hormone receptor, type II (AMHR2) gene, designated AMHR2^cre/cre, to localize its expression. Exon 3 of β-catenin encodes for multiple phosphorylation sites that are necessary for its degradation, while its removal maintains the protein's functionality. However, the excision of exon 3 of Catnb by Cre was a relatively inefficient process as few Catnb^flox(ex3)Amhr2^cre/+ mice displayed abnormal expression of β-catenin. Histochemical analysis showed that the ovaries of 3 to 24-week-old transgenic mice developed abnormal follicle-like structures consisting of pleiomorphic granulosa cells without the presence of an oocyte, resulting in sub-fertility due to an impaired follicular response that could be overcome with age at the end of the third month. GCT were seen at 19 weeks with the incidence of formation over time to 57% at 7.5 months.

Building upon the previous study, Lague et al. 58 conditionally deleted PTEN in the granulosa cells by crossbreeding PTEN^flox/floxwith AMHR2^cre/cre mice to create PTEN^flox/floxAMHR^cre/+. Most PTEN^flox/floxAMHR^cre/+ mice failed to generate any ovarian abnormalities; while these animals could establish pregnancies, they failed to carry the litter to term or had small litters due to fetal death. However, 5 of 70 (~ 7%) female PTEN^flox/floxAMHR^cre/+ developed ovarian tumors. Four of the 5 were bilateral tumors developing between 7 weeks and 7 months that were identified as GCT. PTEN^flox/floxAMHR^cre/+ mice also developed tumor cell emboli and metastases in the lungs. PTEN^flox/floxAMHR^cre/+ GCT showed altered PI3K/Akt signaling, with increases in both phosphorylated Akt and mammalian target of rapamycin (mTOR) levels compared to normal granulosa cells. Furthermore, to determine if the PI3K/Akt pathway could cross-talk with the WNT/CTNNB1 (encoding β-catenin) pathway, they constitutively activated both pathways using the mouse model PTEN^flox/floxCTNNB1^flox(ex3)AMHR^cre/+. These mice developed bilaterial ovarian tumors with 100% penetrance at an early age. Dysplastic cells were seen in the ovaries of newborn mice and 20.5-day embryos suggesting that this occurs perinatally. The ovarian tumors visibly distended the abdomen by 5 weeks of age with death occurring before 9 weeks, possibly due to severe anemia. Pulmonary emboli were also seen in PTEN^flox/floxCTNNB1^flox(ex3)AMHR^cre/+ mice.